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biotinylated histone peptides  (MedChemExpress)
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MedChemExpress biotinylated histone peptides
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Biotinylated Histone Peptides, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated histone 4 peptide [lys(5,8,12,16)ac]-h4(1-21)-biotin  (AnaSpec)
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AnaSpec biotinylated histone 4 peptide [lys(5,8,12,16)ac]-h4(1-21)-biotin
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Biotinylated Histone 4 Peptide [Lys(5,8,12,16)Ac] H4(1 21) Biotin, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemically synthesized c-terminally biotinylated histone h3 peptides bearing the modifications of interest  (Aushon Biosystems)
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Aushon Biosystems chemically synthesized c-terminally biotinylated histone h3 peptides bearing the modifications of interest
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Chemically Synthesized C Terminally Biotinylated Histone H3 Peptides Bearing The Modifications Of Interest, supplied by Aushon Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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histone h3k9me3 peptide, biotinylated  (Active Motif)
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Active Motif histone h3k9me3 peptide, biotinylated
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Histone H3k9me3 Peptide, Biotinylated, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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histone h3k27me3 peptide, biotinylated  (Active Motif)
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Active Motif histone h3k27me3 peptide, biotinylated
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Histone H3k27me3 Peptide, Biotinylated, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated histone peptides h3 (1-21)  (SciLight Biotechnology LLC)
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SciLight Biotechnology LLC biotinylated histone peptides h3 (1-21)
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Biotinylated Histone Peptides H3 (1 21), supplied by SciLight Biotechnology LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated histone h3k4 and h3k27 trimethylated (me3) peptides and relative unmodified (me0) motifs  (Active Motif)
90
Active Motif biotinylated histone h3k4 and h3k27 trimethylated (me3) peptides and relative unmodified (me0) motifs
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Biotinylated Histone H3k4 And H3k27 Trimethylated (Me3) Peptides And Relative Unmodified (Me0) Motifs, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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histone h3 (1−21) lysine 9 mono -methylated biotinylated peptide (# 64358)  (AnaSpec)
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AnaSpec histone h3 (1−21) lysine 9 mono -methylated biotinylated peptide (# 64358)
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Histone H3 (1−21) Lysine 9 Mono Methylated Biotinylated Peptide (# 64358), supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated histone h3k4 h3k27 trimethylated (me3) peptides  (Active Motif)
90
Active Motif biotinylated histone h3k4 h3k27 trimethylated (me3) peptides
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
Biotinylated Histone H3k4 H3k27 Trimethylated (Me3) Peptides, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c-terminal biotinylated tetra-acetylated histone peptide h4 (bh4kac4)  (GenScript corporation)
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GenScript corporation c-terminal biotinylated tetra-acetylated histone peptide h4 (bh4kac4)
a Pulldown assays using MBP-Eaf3 and <t>biotinylated</t> histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.
C Terminal Biotinylated Tetra Acetylated Histone Peptide H4 (Bh4kac4), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Pulldown assays using MBP-Eaf3 and biotinylated histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.

Journal: Nature Communications

Article Title: A repressive H3K36me2 reader mediates Polycomb silencing

doi: 10.1038/s41467-024-51789-6

Figure Lengend Snippet: a Pulldown assays using MBP-Eaf3 and biotinylated histone peptides carrying the indicated methylation. Two independent experiments had similar results. b Pull-down assays using truncated Eaf3 and biotinylated histone peptides carrying the indicated methylation. c MST measuring the affinity of binding between MBP-Eaf3 and peptides with H3K36, H3K27, H3K9 and H3K4 methylation. Kd, dissociation constant. NBD, no binding detected. Data points are represented as means ± SD from three independent replicates. d A Venn diagram showing significant overlap among gene sets of Ash1-H3K36me2, H3K27me3, and Eaf3. The significance of overlap was determined using two-sided Fisher’s exact test. e Relative abundance of H3K27me3 in the indicated strains. Relative abundance of H3K27me3 to H3 was calculated by ImageJ software and that of WT was set as 1. Values are means ± SD from five biological replicates. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values and western blots are shown in the Source Data. f A Venn diagram showing the significant overlap between gene sets of H3K27me3 (WT/∆ kmt6 ) and H3K27me3 (∆ eaf3 /∆ kmt6 )). P -values were calculated by two-sided Fisher’s exact test. g , h Heatmaps and metagene plots showing H3K27me3 ChIP-seq signals of the H3K27me3-marked regions in the WT and ∆eaf3 strains. i Box plots and violin plots showing average transcriptions levels of the genes with and not H3K27me3 in the indicated strains. For box-plot, the horizontal lines from top to bottom represent the maximum, first quartile, median, third quartile, and minimum of the total data respectively. Statistical significance was assessed by a two-tailed Student’s test (** P < 0.01). The exact P -values are shown in the Source Data. For g and h , metaplots and heatmaps are shown with one biological replicate of ChIP-seq or RNA-seq assays, and similar results were gained in other replicates.

Article Snippet: Biotinylated histone peptides were incubated with magnetic streptavidin beads (MedChemExpress, HY-K0208) in a binding buffer (20 mM Tris-HCl pH 7.9, 0.2 mM EDTA, 1 mM DTT, 0.2 mM PMSF, 20% Glycerol, 200 mM KCl and 1 × cocktail proteinase inhibitor freshly) for 4 h at 4 °C.

Techniques: Methylation, Binding Assay, Software, Two Tailed Test, Western Blot, ChIP-sequencing, RNA Sequencing